Search results for " cation exchange"
Poster
Comparison of Three Different Screening Techniques for Predicting Step Elution Conditions for a Cation Exchanger
The aim of this study was to investigate conditions for reduction of host cell proteins (HCP) on a strong cation exchanger, Capto™ S ImpAct. Three different screening techniques using PreDictor™ plate…
Article
Evolution of the Monoclonal Antibody Purification Platform
• Cation exchange (CEX) chromatography is also quite commonly used. The primary objective of this step is to remove host-cell proteins, DNA, charged variants, and aggregates. Bind and elute mode is us…
Article
Development of Purification for Challenging Fc-Fusion Proteins
…Fc-fusion protein polishing process that incorporates flow-through strategy in polishing steps using cation exchange or hydrophobic interaction chromatography.
As tested on three Fc-fusi…
Article
Top Tips for Successful Development of Antibody Chromatography Processes
So, a cation exchange step and an anion exchange step. If we look at this process, the typical step that removes aggregates is the cation exchange step. So let's leave that for now and look at the ani…
Article
The Development and Application of a Monoclonal Antibody Purification Platform
The eluate was then held at this low pH to inactivate viruses, and then adjusted to increase the pH before the cation exchange chromatography step.
After cation exchange, diafiltration was perf…
Article
Technology Innovations Improve Process Chromatography Performance
One area of bottlenecking in continuous mAb polishing chromatography is the cation exchange step, which is traditionally operated in bind and elute mode to provide clearance of product-related aggrega…
Article
Process Chromatography: Continuous Optimization
Polishing is then achieved using some combination of anion exchange (AEX), cation exchange (CEX), mixed mode, and HIC resins.
“Protein-A based affinity chromatography is very popular and more wide…
Article
Process Chromatography Selection for Downstream Processing Applications
However, there are mAb purification processes that utilize cation exchange (CEX) chromatography as the capture step instead of Protein A. For example, Tao et al. (2) evaluated several high-capacity CE…
Article
Addressing the Challenges in Downstream Processing Today and Tomorrow
Anion exchange, cation exchange, and HIC resins were screened in 96 well plates in an HTS format. Additionally, ceramic hydroxyapatite (CHA), immobilized metal affinity chromatography (IMAC), and HIC …
Article
Optimizing Cation-Exchange Chromatography with High-Throughput Process Development for mAb Purification
Even this platform process requires some fine-tuning and adjustment for new mAb targets, especially at the first polishing step with cation exchange chromatography in bind-and-elute mode. This step of…