Search results for " cation exchange"

Poster Polishing of monoclonal antibodies using a polymer grafted cation exchanger
A typical monoclonal antibody (MAb) purification process begins with capture on a protein A chromatography medium (resin), followed by polishing steps by for example ion exchange chromatography. The …

Poster Polishing of Monoclonal Antibodies Using a New Cation Exchanger Capto™ S ImpAct
This application note present results from a study using Capto™ S ImpAct, a new, strong cation exchanger designed for polishing of MAbs. Click to download the application note.

Article Purification Strategies to Process 5 g/L Titers of Monoclonal Antibodies
Depending on the platform, this is followed by either the cation exchange chromatography step in bind-and-elute mode3 or the anion exchange chromatography step in flow-through mode. The virus filtrati…

Article Evolution of the Monoclonal Antibody Purification Platform
Cation exchange (CEX) chromatography is also quite commonly used. The primary objective of this step is to remove host-cell proteins, DNA, charged variants, and aggregates. Bind and elute mode is us…

Article Development of Purification for Challenging Fc-Fusion Proteins
…Fc-fusion protein polishing process that incorporates flow-through strategy in polishing steps using cation exchange or hydrophobic interaction chromatography. As tested on three Fc-fusi…

Article The Development and Application of a Monoclonal Antibody Purification Platform
The eluate was then held at this low pH to inactivate viruses, and then adjusted to increase the pH before the cation exchange chromatography step. After cation exchange, diafiltration was perf…

Article Process Chromatography: Continuous Optimization
Polishing is then achieved using some combination of anion exchange (AEX), cation exchange (CEX), mixed mode, and HIC resins. “Protein-A based affinity chromatography is very popular and more wide…

Article Bioterminology: A Guide to the Biopharmaceutical Lexicon
It is a cation exchange resin.  CMC Chemistry, manufacturing, and controls; the section of a BLA, NDA, or IND describing the composition, manufacture, and specifications of a drug product and its …

Article Mixed-Mode Chromatography in Downstream Process Development
The amino groups of proteins bind to the phosphate ions of the mineral by a classical cation exchange mechanism, and elution is typically achieved by increasing salt or phosphate buffer concentration.…

Article Process Chromatography Selection for Downstream Processing Applications
However, there are mAb purification processes that utilize cation exchange (CEX) chromatography as the capture step instead of Protein A. For example, Tao et al. (2) evaluated several high-capacity CE…

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