Search results for " protein"

Article Re-use of Protein A Resin: Fouling and Economics
Bracewell BioPharm International Volume 3, Issue 28 In the past two decades, Protein A affinity chromatography has remained the de facto capture step for purification of monoclonal antibod…

Article Optimization of Protein Expression in Escherichia Coli
Many problems are associated with protein expression, including toxicity, misfolding and degradation, aggregation in inclusion bodies, low yields, and difficulties in purification. One of the most com…

Article Development of Purification for Challenging Fc-Fusion Proteins
Particularly for Fc-fusion proteins, these improvements have resulted in a new set of starting conditions for downstream processing, including elevated levels of high molecular weight species (HMWs; u…

Article Detecting Protein Aggregates and Evaluating their Immunogenicity
Protein aggregation can occur during all stages of the lifetime of a protein therapeutic, including expression, refolding, purification, sterilization, shipping, storage, and delivery processes (1). T…

Article Fusion Proteins Pose Manufacturability Challenges
Challener Fusion proteins are engineered proteins that contain domains from different proteins that originate from different sources, such as a cell surface receptor or cytokine (i.e., interferon …

Article Host-Cell Protein Measurement and Control
HCPs are proteins produced or encoded by the host organisms used to produce recombinant therapeutic proteins (1). Recombinant therapeutic proteins are usually produced by genetically-modified prokaryo…

Article Predicting Progress in Protein Aggregation
…xchange mass spectrometry (HDX MS), are enabling the improved design, production, and formulation of protein-based drugs. As the development of biologic drugs based on antibodies and other protein/pe…

Article Implications of Cell Culture Conditions on Protein Glycosylation
Glycosylation can potentially affect a protein’s half-life, immunogenicity, binding activity, and stability. It is a complex process that consists of the attachment of carbohydrate moieties, with poss…

Article Analyzing Proteins Using SEC, MALS, and UHPLC
The characterization of proteins involves identification of their complex chemical and physical structures and requires the use of a range of analytical techniques. Size exclusion chromatography (SEC)…

Article Challenges of Protein Aggregation During Purification
32-35  Removal of protein aggregates from biologic APIs is crucial due to their potential to increase immunogenicity. While most aggregates are formed during upstream operations, and the risk for …

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