Search results for " host cell"
Article
Host-Cell Protein Measurement and Control
Recombinant therapeutic proteins are usually produced by genetically-modified prokaryotic or eukaryotic host cells using cell culture/fermentation technology (2). Genetic engineering allows the host c…
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Overview of a scale-up of a cell-based influenza virus production process using ReadyToProcess equipment
Impurities originating from the host cells were removed during the downstream process. Cell debris was removed in the first microfiltration step using ULTA Prime GF 2 μm and 0.6 μm normal flow filters…
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Gene Therapies Push Viral Vector Production
Lentiviral vectors have displaced traditional retroviral vectors because lentiviral vectors do not require dividing host cells for integration, Carstens further explains. This characteristic broadens …
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Improving Upstream Predictability
Over the past three years, Mohindra adds, Lonza has significantly improved host cell expression levels along with its understanding of the design space and interactions between cells, media components…
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Platform Approach Speeds Process Development
…ch as recombinant monoclonal antibodies is dependent on high performing bioprocesses using mammalian host cells. Chinese hamster ovary (CHO)-based cell culture platforms should combine state-of the-a…
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Impurity Testing of Biologic Drug Products
Some impurities (especially host cell proteins) may add an immune-stimulating or adjuvant behavior to the therapeutic, causing the patient to generate antibodies or cell-mediated immunity against the …
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Platform Technologies Improve Protein Expression
Understanding and tools for better engineering the overall metabolism of host cells with the capability to balance protein generation in several different phases with cell growth and survival will rem…
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Addressing the Challenges in Downstream Processing Today and Tomorrow
In addition, the HIC step reduced the level of host cell proteins by 1.4 log10 . Based on these data, the final process used a Protein A capture step with elution at low pH followed by immediate neutr…
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Quality by design for biotechnology products—part 1
Examples of process-related impurities include host cell DNA, host cell protein, adventitious agents, residual medium supplements such as protein hydrolysates, and components such as nucleases or resi…
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Combining Microbioreactors and Advanced Statistical Techniques to Optimize a Platform Process for a New Host-Cell Line
Use of a subspace model is a viable method to characterize process space variables and optimize process performance.
By Colin Jaques, Daniela Lega
Over the past decade, two improved capabili…