Search results for " capture" in Articles / App Notes
Article
A deep dive into optimizing AAV capture and polishing to maximize both yield and purity
To meet these needs, Cytiva developed scalable capture and polishing chromatography steps for recombinant AAV (rAAV). The affinity capture step maximizes recovery and efficiently removes impurities.
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Strategies and Emerging Technologies for mAb Capture
Strategies will depend on the mode of upstream operations (fed batch or perfusion) and the desired process improvement of the capture step, (e.g., productivity, cost, or process time) as well as the t…
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Optimizing capture and polishing steps in an rAAV purification process
The chromatography purification described includes affinity capture to maximize recovery with efficient impurity removal.
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Capture of Bispecific Antibodies and Removal of Product-Related Impurities
One potential solution is to initiate polishing at the capture step using differences in avidity. In this article, Cytiva presents data on applications using MabSelect™ VL protein L resin with novel …
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Case Study: Virus Reduction of an Affinity Capture Step
In this study, the virus reduction of an affinity capture step was evaluated in a scale-down column format. The study was performed by a third-party viral testing laboratory in collaboration with a bi…
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Multidimensional Scale-Up of a Monoclonal Antibody Capture Step, Using ÄKTA™ pilot 600
In this work, a multidimensional scale-up (change of both column diameter and bed height) of a mAb capture step, using the ÄKTA pilot 600 chromatography system, is demonstrated. Column volume per…
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Process Economy Simulations on a Protein A Capture Step
MabSelect PrismA is a next‐generation protein A chromatography resin that offers significantly enhanced alkaline stability and capacity compared with its predecessor products. This application note …
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Evolution of the Monoclonal Antibody Purification Platform
Typical steps include:
• Protein A chromatography for capture of the product and removal of host cell-related impurities (host cell proteins and DNA). Though extremely efficient and effective,…
Article
Successfully Moving Regulated Data to the Cloud
The document provides clarification on how data should be captured to most effectively preserve its integrity, primarily in the current good manufacturing practice (CGMP) regulated space (1).
The d…
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Digitalization: The Route to Biopharma 4.0
In two-column capture chromatography, dynamic control can be obtained using adaptive model-based control strategy that led to process yield of more than 85%. The data from high performance liquid chro…