Search results for " Hydrophobic interaction chromatogr…"
Article
Increasing Productivity in Hydrophobic Interaction Chromatography (HIC) Using Capto™ Resins
In this study, the hydrophobicities of newer-generation Capto™ HIC resins were compared to Sepharose™ High Performance and Sepharose Fast Flow HIC resins. An application example is also present…
Article
Adsorption isotherm models for chromatography
Read on commonly used models for the thermodynamics of adsorption within a chromatography column.
Indeed, in order to create a complete chromatography model, the adsorption of compon…
Poster
Guide to modern BioProcess™ chromatography resins
Proteins are different. Based on what protein you would like to purify and the conditions used, chromatography resins are selected to optimize purity and yield. Many bioprocess applications include …
Poster
A Next Generation Protein A Chromatography Resin for Improved Productivity and Bioburden Control
This poster presents a newly designed, high-capacity Protein A resin, MabSelect™ PrismA, which offers alkali stability on par with ion exchange and hydrophobic interaction chromatography resins, enabl…
Article
Evolution of the Monoclonal Antibody Purification Platform
This can result in a different selectivity than what is offered by traditional ion-exchange or hydrophobic interaction chromatography, thus offering a larger range of ionic strength and pH under which…
Article
Development of Purification for Challenging Fc-Fusion Proteins
…olishing process that incorporates flow-through strategy in polishing steps using cation exchange or hydrophobic interaction chromatography.
As tested on three Fc-fusion proteins, this p…
Article
Challenges of Protein Aggregation During Purification
Careful selection of downstream processing conditions is a must.
Sep 1, 2014
By: Cynthia Challener, PhD
BioPharm International
Volume 27, Issue 9, pp. 32-35
Removal of protein a…
Article
A Closer Look at Affinity Ligands
Affinity ligands play an exciting role in the ever-growing sphere of downstream processing. In speaking with BioPharm International®, Phillip Elliott, PhD, associate director of Process Development,…
Article
SEC in the Modern Downstream Purification Process
Mar 01, 2015
By R. Christopher Manzari, J. Kevin O'Donnell
BioPharm International
Volume 3, Issue 28
Since the introduction of commercial chromatography resins approximately six de…
Article
Enhancing Resins Addresses Purification Concerns
Deorkar notes that hydrophobic interaction chromatography (HIC) and anion exchange (AEX) chromatography can be used, but careful optimization of conditions is required to be applicable for large-scale…