Search results for "size exclusion chromatography"
Article
HT Multi-Product Liquid Chromatography for Characterization of Monoclonal Antibodies
Many of the recommended protein characterization assays are based on liquid chromatography methods such as ion exchange chromatography (IEC) for charge heterogeneity analysis, size exclusion chromatog…
Article
Process Chromatography Selection for Downstream Processing Applications
Industry experts discuss best practices for selecting a separation technology.
By Caroline Hroncich
Process chromatography has changed significantly since it was first introduced to the biop…
Article
Selection Guide Size Exclusion Chromatography Columns and Media
Size exclusion chromatography (SEC), also called Gel filtration (GF), separates molecules on the basis of differences in size as they pass through a SEC medium packed in a column. A variety of media…
Article
Exosome isolation by tangential flow filtration and size exclusion chromatography
The workflows we describe are suitable for scale-up and combine tangential flow filtration (TFF) for the concentration of exosomes followed by gentle size exclusion chromatography (SEC) with our next-…
Article
Selectivity in Size Exclusion Chromatography (SEC): It’s Much More than Average Pore Diameter (Å)
Describing the pore of a size exclusion chromatography resin with a single number such as average pore diameter is an oversimplification. The formation of pores is dependent on complex combinations of…
Article
Protein Analysis with Size Exclusion Chromatography
Size exclusion chromatography (SEC) is widely used in several different analytical applications from basic research to quality control of biotherapeutics. This white paper presents fundamentals abou…
Article
Handbook: Size Exclusion Chromatography Principles and Methods
This handbook describes the use of SEC for the purification and separation of biomolecules, with a focus on practical information for obtaining the best results. The media available, selection crite…
Article
SEC in the Modern Downstream Purification Process
Mar 01, 2015
By R. Christopher Manzari, J. Kevin O'Donnell
BioPharm International
Volume 3, Issue 28
Since the introduction of commercial chromatography resins approximately six de…
Article
Evolution of the Monoclonal Antibody Purification Platform
The authors discuss the evolution of the purification platform for manufacturing of mAb therapeutics.
Monoclonal antibodies (mAb) increasingly form the majority share of the product pipeline…
Article
Analyzing Proteins Using SEC, MALS, and UHPLC
Size exclusion chromatography (SEC) is generally used as a non-denaturing method that provides information on the apparent molecular weight, the presence of aggregates or higher molecular weight (MW) …