Search results for "plasmids"

Article Scaling Up an E. coli Upstream Process for Plasmid DNA Production — from 2.5 to 160 L scale Fermenters
Read more >> This article summarizes the results of Escherichia coli growth performance for plasmid production in a 5 L glass fermentor, which was scaled up to 80 and 160 L scale in Xcellerex™ X…

Article Improving Capacity in DNA Plasmid Purification
Working with plasmids? Plasmid supply is in high demand these days. Join Tony Hitchcock on May 6, 2020 to learn more about this critical market and how Cobra Biologics is working with Cytiva …

Article Purification of Plasmid DNA
Plasmid DNA is the building block of advanced therapies. As these therapies take off, so does manufacturing demand. By switching to modern chromatography resins, Cobra Biologics was able to increase…

Article Akron Bio Will Install a Cytiva FlexFactory to Manufacture Plasmid DNA
Cytiva will provide the first FlexFactory to be used in the manufacture of pDNA to Akron Bio. Cytiva will provide its FlexFactory, a flexible, single-use platform, to enable Akron Biotech to m…

Article A Risk-Based Genetic Characterization Strategy for Recombinant CHO Cell Lines Used for Clinical and Commercial Applications
The authors describe the ways in which manufacturers can mitigate the risks related to the integrity of recombinant transgenes expressed in CHO cells. By Luhong He, Christopher Frye Abstract …

Article Gene Therapies Push Viral Vector Production
Plasmids can be easily modified to deliver a therapeutic gene and optimize its expression in a host cell. The incorporation of a multiple cloning site into a plasmid enables the insertion of a gene o…

Article Optimization of Protein Expression in Escherichia Coli
… efficient repression, the plasmid must also carry the lacI or lacIq gene, especially for high copy plasmids. The lacIq gene contains a mutation in its promoter that enhances lacI expression by tenfo…

Article Scalable Viral Vector Manufacturing
…iated Virus, the virus can be produced upon introduction into the host cell line by transfection of plasmids encoding both the viral genes and the gene of interest. Thus, the plasmids are used as a r…

Article Enhancing Resins Addresses Purification Concerns
Christoph Burgstedt/Stock.Adobe.com Enhancement of resin technology can improve the ability of chromatography to purify the growing landscape of increasingly complex biomolecu…

Article Working With Advanced Therapeutics: You Bring the Idea, We Bring the Map
Plasmids. mRNA. Oligos. Cell and gene therapies. Antibody variants. Vaccines. We all know these advanced therapies hold huge potential. But developing them isn’t straightforward, and fai…

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