Search results for " yield"

Article Combine Chromatography Techniques: Get the Right Purity/Yield of Protein
This attractive wall poster shows you how to effectively combine techniques and succeed in your protein purification.    Order now for free  

Poster Optimization of product quality and yield in high-performing fed-batch CHO cell cultures
This poster summarizes data from studies of product quality of a human monoclonal antibody (IgG1) produced from Chinese hamster ovary (CHO) cells cultured in fed-batch mode.

Poster High yield antibody production in a disposable WAVE Bioreactor™
Introduction Perfusion processes provide high volumetric productivity by retaining a large cell number in a relatively small volume. These characteristics can be used for process intensificati…

Article Developing a mAb aggregate removal step by high throughput process development
The use of the 96-well plate format enabled the efficient screening of three different media and optimization of chromatographic parameters to maximize yield at the desired purity level. Biopharmace…

Article Polishing of monoclonal antibodies using Capto adhere ImpRes in bind and elute mode
The results showed high yields of monomeric MAb, as well as good clearance of aggregates, host cell proteins (HCP), and leached protein A.    Introduction MAbs and MAb conjugates are today …

Article A platform approach for the purification of antibody fragments (Fabs)
Using Capto™ L, Capto SP ImpRes, and Capto Q media in the three step process resulted in efficient removal of the main contaminants and high yields (≥ 87%) over the entire process. This platform appro…

Article Optimization of Protein Expression in Escherichia Coli
Many problems are associated with protein expression, including toxicity, misfolding and degradation, aggregation in inclusion bodies, low yields, and difficulties in purification. One of the most com…

Article Economic drivers and trade-offs in antibody purification processes
Consequently, the downstream yield and material costs become significant cost drivers. DSP bottlenecks can lead to increased investment in larger equipment and longer batch durations. This results in …

Article Faster scale-up of a two-step MAb process with AxiChrom™ columns and ReadyToProcess™ solutions
These filtration steps were also optimized by determining robust parameters that consistently produced high yields. Capture step Target MAb was expressed in CHO cells cultured in a 120 l stirr…

Article Development of Purification for Challenging Fc-Fusion Proteins
As tested on three Fc-fusion proteins, this process demonstrates high process yield, improved clearance of HMWs and LMWs, and efficient reduction of host cell proteins (HCP) and DNA as well as robust …

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