Search results for " yield"
Article
Addressing the Challenges in Downstream Processing Today and Tomorrow
coli expression often yields high levels of protein production, often in the form of inclusion bodies, which require extra steps involving their isolation and the subsequent extraction and refolding t…
Article
Science Focus Fuels Successful Process Development for Startups
Optimizing cell yield is crucial in cell therapies because cell yield has a direct impact on the quantity of cells available for administration to the patient. “Unlike with batch processes where maxim…
Article
Best Practices for Data Integrity
A thought process, for example, may be ‘Now we have production yield and quality data that are reliably collected, and we can trust their integrity. How might we leverage that data to minimize variabi…
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Designing a Biomanufacturing Facility Incorporating Single-Use Technologies
A typical batch at 2 g/L with a 70% overall yield in downstream processing and a 95% production success rate will therefore yield 138 kg/yr in total. The final yield here is determined by the product …
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Bioprocessing Advances in Vaccine Manufacture
The BioPlan annual survey of bioprocessing professionals and other studies show a rather consistent doubling of mammalian-cell protein expression and product yield about every five years, with yields …
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Cell-free Expression Systems Pose Cell Culture Alternative
The professors point to studies of these technical advances that have shown the following results:
Protein yields exceed grams of protein per liter of reaction volume; batch reactions last mult…
Article
Improving Upstream Predictability
By Agnes Shanley
In the earliest days of biotech, developers often struggled to sustain conditions that would optimize cell health and product yield. Today, better understanding of cells and …
Article
Platform Technologies Improve Protein Expression
…ression that will not only facilitate the development of biologic drug candidates, but also increase yield and speed to market.
A platform of benefits
Platform technologies for protein ex…
Article
Optimizing Cation-Exchange Chromatography with High-Throughput Process Development for mAb Purification
Aggregate concentration and host-cell protein (HCP) levels in the eluate at a 90% monomer yield were observed. The loading pH was found to have a significant effect on aggregate concentration, where l…
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Comparing Protein A Resins for Monoclonal Antibody Purification
Protein A is the affinity chromatography ligand of choice for the first antibody capture step, because its high selectivity gives excellent purity (typically > 99%) and high yields. Furthermore, Prote…