Search results for " ion exchange"
Article
Process Chromatography Selection for Downstream Processing Applications
In cases where ion exchangers cannot provide the required separation, hydrophobic interaction and mixed-mode adsorbents are generally used, although in some cases, these may require more effort to opt…
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HT Multi-Product Liquid Chromatography for Characterization of Monoclonal Antibodies
Many of the recommended protein characterization assays are based on liquid chromatography methods such as ion exchange chromatography (IEC) for charge heterogeneity analysis, size exclusion chromatog…
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Predicting Progress in Protein Aggregation
Techniques to enable the design and formulation of stable, protein-based therapeutics.
In silico analysis and the evaluation of formulability, aided by new analytical tools such as hydrogen deut…
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Top challenges in recombinant protein purification process development
So after your affinity step, you can use a combination of ion exchangers and the monoclonal antibodies will react quite similarly each time, so this platform approach can be used. Whereas for recombin…
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Modeling the Degradation of mAb Therapeutics
Modeling the Degradation of mAb Therapeutics
Kinetic models can be used to study aggregation and fragmentation to help ensure stability.
By Anurag S. Rathore, Rohit Bansal
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The Challenge of Disruptive Technologies in Bioprocessing
Next-generation ion exchange chromatography resins like POROS XS (strong cation exchange) and XQ (strong anion exchange) offer high capacity, high resolution, and salt tolerance at faster flow rates f…
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Improving Process-Scale Chromatography
Alternatives to Protein A chromatography are available based on techniques such as precipitation, ion exchangers, and multimodals/mimetics. “It is challenging to compete on selectivity with the years …
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Elucidating Biosimilars Characterization
The global market for biosimilar drugs has been forecasted to be $2.445 billion in 2013 according to a report by the British market-research firm, Visiongain (1). The growth corresponds to a …
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Enhancing Resins Addresses Purification Concerns
The most common large-scale process for purification of mRNA, for example, is affinity chromatography followed by ion exchange (IEX) or reverse phase (RP) chromatography (1). In lab-scale processing, …
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How Coupling MALS to IEX Can Help Protein Characterization
Coupling ion exchange chromatography (IEX) to multi-angle light scattering (MALS) can open new opportunities in protein characterization. Mario Lebendiker and Hadar Amartely from The Hebrew University…