Search results for " plates"
High-throughput screening of HIC media in PreDictor™ plates for capturing a recombinant protein from E.coli
A parallel screening of HIC media and conditions in 96-well plates facilitates the selection of the most promising HIC media and shows how salt type and salt concentration conditions could be tailored…
Buffer plate preparation using ÄKTA™ avant system and BufferPro tool
A method was developed for automatic buffer preparation of 48-well buffer plates using ÄKTA avant chromatography system and the BufferPro tool in UNICORN™ 6 software.Using BufferPro with ÄKT…
Mini-columns as a Complement to 96-well Filter Plates in a Process Development Workflow
96-well filter plates and mini-columns packed with chromatography media (resins) are useful tools for process development.
High-throughput screening of HIC media in PreDictor™ plates for capturing recombinant Green Fluorescent Protein from E. coli
This study demonstrates a rapid, high-throughput process development workflow using parallel screening in 96-well plates to determine the most suitable HIC media and conditions for capturing recombina…
PreDictor™ plates and Assist software
Using PreDictor™ plates and Assist software helps to increase the efficiency of process development.
Developing a mAb aggregate removal step by high throughput process development
These experiments were performed using 96-well plates (PreDictor, GE Healthcare) filled with the three chromatography media being studied, which eliminated the need for the more time-consuming column …
High-throughput process development and scale-up of an intermediate purification step for recombinant insulin
Conditions defined using PreDictor plates can be verified and optimized in column format with HiScreen™ or HiTrap™ prepacked columns. An automated chromatography system such as AKTA avant 25 wil…
Development of Purification for Challenging Fc-Fusion Proteins
In this study, utilizing a combination of high-throughput condition screening in 96-well plates, optimization in small-scale column models, and a novel virus-spiking strategy, the authors have develop…
Tools for Continuous Bioprocessing Development
For instance, in cell culture, the typical biopharmaceutical company uses at least a dozen different platforms, including shallow-well microtiter plates; deep-well microtiter plates; shake flasks; ben…
Optimizing Cation-Exchange Chromatography with High-Throughput Process Development for mAb Purification
An example illustrating the use of HTPD and DoE to optimize the first polishing step for a mAb of interest is discussed herein, and the roles of both HTPD 96-well filter plates and mini-columns are hi…