Search results for " cell growth"

Article A Risk-Based Genetic Characterization Strategy for Recombinant CHO Cell Lines Used for Clinical and Commercial Applications
The authors describe the ways in which manufacturers can mitigate the risks related to the integrity of recombinant transgenes expressed in CHO cells. By Luhong He, Christopher Frye Abstract …

Article Adherent Cell Culture in Biopharmaceutical Applications: The Cell-Detachment Challenge
The necessity to detach cells from a culture substrate during cell harvesting remains one of the most challenging steps in a cell-culture process. By Marcos Simon, Juan J. Giner-Casares Cell

Article Scale-up of adherent Vero cells grown on Cytodex microcarrierrs using ReadyToProcess equipment
The cell growth was similar in the 10 L seed cultures as in the 50 L production cultures in three consecutive experiments, with an obtained Hemagglutinin (HA) concentration of approximately 12 μg/mL a…

Article Best Practices for Selecting a Top-Quality Cell Line
Factors such as cell growth and metabolism, as well as product quality attributes like glycosylation distribution, are closely related to the culture medium, and thus many cell-culture media component…

Article Mapping a Route for Cell and Gene Therapy Process Development
While cell and gene therapies differ in many ways, some of the best practices for process development and validation are similar. By Cynthia A. Challener …

Article Design Considerations for a Commercial Cell and Gene Therapy Facility
The commercialization of cell and gene therapies has become a reality, prompting deeper considerations of logistics, technology, and design for manufacturing facilities. By Feliza Mirasol …

Article Large-scale cell culture using Cellbag™ bioreactors with embedded optical sensors
This application note describes how WAVE Bioreactor™200 system has been used with a disposable Cellbag 200L with embedded DOOPT II and pHOPT sensors to cultivate cells.  For a download of the complete…

Article Implications of Cell Culture Conditions on Protein Glycosylation
The authors present a review of the techniques commonly used for glycosylation analysis. By Michiel E. Ultee, PhD, Dr. Richard Easton This article reviews the implications of cell-culture co…

Article Trends in Fermentation and Cell Culture Equipment
Process Development Forum spoke with Parrish Galliher, who has been the CEO and founder of Xcellerex, which is now part of GE Healthcare. The discussion focused on future trends in fermentation and ce…

Article Fed-batch supplements to boost cell culture performance
To increase the yield of a target protein, fed-batch culture is often employed in biomanufacturing processes. This application note demonstrates the enhanced performance of commonly used cell lines …

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