Search results for "plasmid"
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Scaling Up an E. coli Upstream Process for Plasmid DNA Production — from 2.5 to 160 L scale Fermenters
coli DH5α strain which produced a pLP2 plasmid of 4180 bp in the study. Performance of peak E. coli growth measured as optical density at 600 nm (OD600), plasmid titer, and percentage of produced supe…
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Improving Capacity in DNA Plasmid Purification
Plasmid supply is in high demand these days. Join Tony Hitchcock on May 6, 2020 to learn more about this critical market and how Cobra Biologics is working with Cytiva to develop a modern chromatograp…
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Modern plasmid purification process
This latest purification technology will offer efficient processing time for plasmid DNA production.
Learn more here.
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Purification of Plasmid DNA
By switching to modern chromatography resins, Cobra Biologics was able to increase binding capacity and flow rates up to 50% to address the increasing demands for high quality plasmid DNA.
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A scalable single-use two-step plasmid purification process
While MAb processes have become much more efficient, production of mRNA and viral vectors is still hindered by the requirement for a reliable source of high-quality plasmid DNA (pDNA), which typically…
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Akron Bio Will Install a Cytiva FlexFactory to Manufacture Plasmid DNA
Cytiva will provide its FlexFactory, a flexible, single-use platform, to enable Akron Biotech to manufacture plasmid DNA (pDNA), which is used in many innovative therapies and vaccines, Cytiva said in…
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A Risk-Based Genetic Characterization Strategy for Recombinant CHO Cell Lines Used for Clinical and Commercial Applications
Early-phase assessment includes characterization of the expression plasmid prior to cell-line generation (transfection); evaluation of transcript integrity of those transgenes expressed transiently an…
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Gene Therapies Push Viral Vector Production
“Plasmids can be easily modified to deliver a therapeutic gene and optimize its expression in a host cell. The incorporation of a multiple cloning site into a plasmid enables the insertion of a gene o…
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Optimization of Protein Expression in Escherichia Coli
The Rosetta strains, which carry a plasmid supplementing tRNAs for six to seven rare codons in E. coli, may help to overcome that. Strains, such as Shuffle (New England Biolabs) and Origami (Novagen),…
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Scalable Viral Vector Manufacturing
How do plasmid manufacturing fit within this increased focus of gene therapy and vaccines?
In some cases, such as for Lentivirus and Adeno-Associated Virus, the virus can be produced upon introd…