Search results for "plasmid"

Article Improving Capacity in DNA Plasmid Purification
Plasmid supply is in high demand these days. Join Tony Hitchcock on May 6, 2020 to learn more about this critical market and how Cobra Biologics is working with Cytiva (formerly GE Life Sciences) to d…

Article Purification of Plasmid DNA
By switching to modern chromatography resins, Cobra Biologics was able to increase binding capacity and flow rates up to 50% to address the increasing demands for high quality plasmid DNA. Click he…

Resource Improving Capacity in DNA Plasmid Purification
Plasmid supply is in high demand these days. Join Tony Hitchcock on May 6, 2020 to learn more about this critical market and how Cobra Biologics is working with Cytiva (formerly GE Life Sciences) to d…

Article A Risk-Based Genetic Characterization Strategy for Recombinant CHO Cell Lines Used for Clinical and Commercial Applications
Early-phase assessment includes characterization of the expression plasmid prior to cell-line generation (transfection); evaluation of transcript integrity of those transgenes expressed transiently an…

Article Bioterminology: A Guide to the Biopharmaceutical Lexicon
cosmid An artificially constructed plasmid vector that contains a specific bacteriophage gene, which allows it to carry up to 45,000 base pairs of desired DNA.  cot1/2 DNA A curve that measures ge…

Article Gene Therapies Push Viral Vector Production
Plasmids can be easily modified to deliver a therapeutic gene and optimize its expression in a host cell. The incorporation of a multiple cloning site into a plasmid enables the insertion of a gene o…

Article Optimization of Protein Expression in Escherichia Coli
The Rosetta strains, which carry a plasmid supplementing tRNAs for six to seven rare codons in E. coli, may help to overcome that. Strains, such as Shuffle (New England Biolabs) and Origami (Novagen),…

Article Scalable Viral Vector Manufacturing
How do plasmid manufacturing fit within this increased focus of gene therapy and vaccines? In some cases, such as for Lentivirus and Adeno-Associated Virus, the virus can be produced upon introd…

Article Best Practices for Selecting a Top-Quality Cell Line
The plasmid ratio and medium are also important, Cai observes. “A balanced plasmid ratio usually has dramatic impact on some formats of bispecific antibody cell line development to minimize impurities…

Article A Look at the Affinity Chromatography Landscape
He points to the use of conventional, non-porous particles in amino acids–DNA affinity chromatography for the purification of super-coiled plasmid DNA (sc pDNA) as an example. “Using amino acid affini…

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