Search results for " screening"
Article
Resolving a bottleneck in screening and characterization of recombinant antibody fragments using Biacore 4000
Biacore 4000 equipped with Biacore 4000 Antibody Extension Package has been used for efficient screening and characterization of antibody fragments for diagnostic purposes. Ten 96-well plates containi…
Poster
Comparison of Three Different Screening Techniques for Predicting Step Elution Conditions for a Cation Exchanger
It is important that the screening can give an indication of the optimal conditions for binding and/or elution in a chromatographic column run. The aim of this study was to investigate conditions for …
Article
Single-Step, Protein A Chromatography Process for Bispecific Antibodies in Early Screening
BsAb constructs were efficiently purified to give more than 90% purity and 85% yield in the early screening downstream process.
Learn more about bispecific purification
Article
Walk Away and Do More Screening Preps
Developing a process or looking for a clone of interest often requires screening large numbers of samples. Many analyses require purified protein, and substantial hands-on time might be allocated to…
Article
Connected, Automated Downstream Process for mAb Screening and Evaluation
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A pre-clinical lab is often faced with the need to constantly evaluate possible ways of increasing capacity and throughput. This study was done to show both proof of p…
Article
Determining Criticality, Part Two: DoE and Data-Driven Criticality
The initial risk assessment provides a screening tool to sort out the parameters that have low or no risk.
Design space and design of experiments
The goal is to increase process knowledge…
Article
Best Practices for Selecting a Top-Quality Cell Line
Two specific challenges, Cai continues, include clippings for bispecific antibody and fusion proteins, which may require many constructs screening and extensive mass analysis or peptide mapping to det…
Article
Development of Purification for Challenging Fc-Fusion Proteins
In this study, utilizing a combination of high-throughput condition screening in 96-well plates, optimization in small-scale column models, and a novel virus-spiking strategy, the authors have develop…
Article
High-Throughput Process Development in an Historical Environment
HTPD approaches in downstream goes beyond chromatography
The approaches in downstream process development, with screening of chromatography resins and parallel screening of optimal process conditi…
Article
Platform Approach Speeds Process Development
The platform includes vector design, cell line engineering, high-throughput screening by automation, systematic design of experiment (DoE)-based approaches for media and cell-culture process developme…