Search results for " pDNA"
Article
Enhancing Resins Addresses Purification Concerns
“For example, the large size of pDNA decreases the capacity of AEX resins due to less accessibility to the pores. In addition, critical impurities, such as open circular pDNA, genomic DNA and high mol…
Article
Akron Bio Will Install a Cytiva FlexFactory to Manufacture Plasmid DNA
Cytiva and Akron will also work together on process design for pDNA production. Akron’s pDNA FlexFactory will be fully operational by the third quarter of 2021, the press release said.
“With thi…
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A Look at the Affinity Chromatography Landscape
He points to the use of conventional, non-porous particles in amino acids–DNA affinity chromatography for the purification of super-coiled plasmid DNA (sc pDNA) as an example. “Using amino acid affini…
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A scalable single-use two-step plasmid purification process
The current global demand for biopharmaceuticals is over USD 300 billion, with an estimated growth of ≥ 12% annually. While MAb processes have become much more efficient, production of mRN…
Article
Single-use Bioreactors Have Reached the Big Time
…ut also dramatically reduce the footprint required to produce the virus titers, and it requires less pDNA to transfect the cells, according to Onraedt. “Single-use bioreactors such as the iCELLis all…