Search results for " microcarrier"

Article Vaccine process development using Cytodex™ microcarriers in WAVE™ Bioreactor systems
In this study, Cytodex microcarriers were used in Cellbag™ bioreactors to expand Madine-Darby Canine Kidney (MDCK) and Vero cells. Different mixing conditions were tested for both cell lines in order …

Poster Virus Production in Single-Use Bioreactor Systems Using Pre-Sterilized Microcarriers
By using ready-to-use Cytodex Gamma microcarriers, the time-consuming step of microcarrier preparation and sterilization prior to culture was removed. Instead, the microcarriers could be added directl…

Article A Microcarrier Cell Culture Handbook
Cell culture techniques are vital to the study of animal cell structure, function and differentiation, and for the production of many important biological materials such as vaccines, enzymes, hormones…

Article Propagation of influenza virus in Vero cells using Cytodex microcarriers in WAVE Bioreactor systems
Finally, the robustness and reproducibility of the entire workflow for virus propagation in Vero cells using microcarriers and WAVE Bioreactor system was investigated. The data show that a robus…

Poster Propagation of influenza virus in Vero cells using Cytodex™ microcarriers in WAVE Bioreactor™ systems
We have performed a study on propagation of live influenza virus in Vero cells by using Cytodex™ microcarriers in WAVE Bioreactor™ systems. Different cell culture media and supplements have been teste…

Poster Animal Component-Free Rotavirus Production Using Microcarriers
Here we present a method in which RV can be propagated in Vero cells expanded on microcarriers using recombinant trypsin and a serum-free cell culture medium. The results demonstrate that RV can be pr…

Article Scale-up of adherent Vero cells grown on Cytodex microcarrierrs using ReadyToProcess equipment
Vero cells were grown to high cell density on Cytodex microcarriers in 10 L working volume. The cells were detached with trypsin and used to seed a 50 L production culture with the same microcarrier c…

Article Downstream Processing for Cell-Based Therapies
Significant progress has been made in recent years with human mesenchymal stem cells (hMSCs) cultured on microcarriers now reaching densities of >1x106 cells mL-1 (4) and human pluripotent stem cells …

Article Overview of a scale-up of a cell-based influenza virus production process using ReadyToProcess equipment
The cells in the 10 L cultures were grown on Cytodex 1 microcarriers (3 g/L) in the WAVE Bioreactor 20/50 system. The cells were grown to a cell concentration of approximately 3 × 106 cells/mL. The ce…

Article Adherent Cell Culture in Biopharmaceutical Applications: The Cell-Detachment Challenge
Degree of cell adhesion Vigorous cell-culture processes, such as culture on microcarriers, require strong cell adhesion that needs be matched by the harshness of the detaching step. On the other h…

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