Search results for " microcarrier"

Article Production of Recombinant Adeno-associated Virus Vectors Using Microcarrier Culture of HEK293T Cells
As of March 2020, more than 4310 gene therapy clinical trials have been performed worldwide, with only 518 of them in Phase III and Phase IV. As the gene therapy projects enter clinical Phase III or g…

Article Downstream Processing for Cell-Based Therapies
Significant progress has been made in recent years with human mesenchymal stem cells (hMSCs) cultured on microcarriers now reaching densities of >1x106 cells mL-1 (4) and human pluripotent stem cells …

Article Overview of a scale-up of a cell-based influenza virus production process using ReadyToProcess equipment
The cells in the 10 L cultures were grown on Cytodex 1 microcarriers (3 g/L) in the WAVE Bioreactor 20/50 system. The cells were grown to a cell concentration of approximately 3 × 106 cells/mL. The ce…

Article Adherent Cell Culture in Biopharmaceutical Applications: The Cell-Detachment Challenge
Degree of cell adhesion Vigorous cell-culture processes, such as culture on microcarriers, require strong cell adhesion that needs be matched by the harshness of the detaching step. On the other h…

Poster Validation of the Production of Influenza Virus
To reduce the need for microcarrier preparation prior to use, presterilized and ready-to-use Cytodex Gamma microcarriers were evaluated and compared with Cytodex microcarriers. The results show compar…

Article Validation of the production of influenza virus in ReadyToProcess WAVE™ 25 bioreactor system
To reduce the need for microcarrier preparation prior to use, presterilized and ready-to-use Cytodex Gamma microcarriers was evaluated and compared with Cytodex microcarriers. The results show compara…

Article Single-Use Technologies Prove Effective for Viral Vector Process Development
Use of microcarriers that support adherent cell lines is also being explored as a means for scaling adherent processes in suspension in stirred-tank bioreactors. Flatware is often used in the seed…

Article Scalable ADCF Virus Production Using HyClone™ VaccineXpress Medium
Small-scale microcarrier cultures were optimized using spinner flasks by closely monitoring cell growth, viability, and morphology. The results were used for scaling up the process to a ReadyToProcess…

Poster Validation of Cytodex™ Gamma microcarrier-based virus production in single-use bioreactor systems
Here, we describe different approaches for the cultivation of Vero cells using Cytodex and Cytodex Gamma microcarriers in serum-free cultivation medium using spinners, WAVE Bioreactor™ 20/50, ReadyToP…

Article Flexible Facilities for Viral Vector Manufacturing
Another option would be to use adherent cells with microcarriers in stirred-tank bioreactors. Both options typically do not have scale limitations and thus would allow for a more scalable process. …

Next Page