Search results for " polishing"

Article Guide to Polishing Chromatography in Process Development
READ THE FULL ARTICLE >> This article shares the factors you should consider when developing your polishing steps—from the basics to the typical challenges faced, and how to overcome them. …

Article HTPD: Optimizing a mAb Chromatography Polishing Step
In this webinar, a case study using high throughput process development tools, optimizing process conditions for a mAb polishing step using a multimodal chromatography resin (Capto MMC ImpRes), will b…

Article Technical and Process Economical Aspects of Using Capto™ Q and ReadyToProcess™Adsorber Q in mAb Polishing
Here, we compare the performance of the disposable ReadyToProcess Adsorber Q membrane capsule with Capto Q resin packed in conventional chromatography columns when used in the final polishing step of …

Poster Optimizing dynamic binding capacity and aggregate clearance in an mAb polishing step
Capto™ S ImpAct is a strong cation exchange (CIEX) chromatography medium (resin) designed for monoclonal antibody (MAb) polishing steps. In this study, optimization of binding conditions targeting h…

Article Optimization of an antibody polishing step
Antibody purification processes are typically developed using a platform approach. Optimal operating conditions for single units are found by altering just a few process parameters with De…

Article Connected Polishing and Concentration Under One Automation Method
Here, the ÄKTA™ ready chromatography system was directly connected to the ÄKTA readyflux filtration system, for polishing and concentration of a target molecule. One automation method was cr…

Article Optimizing capture and polishing steps in an rAAV purification process
In this article, we show how we developed an efficient purification process for recombinant AAV (rAAV). The chromatography purification described includes affinity capture to maxi…

Article Development of Purification for Challenging Fc-Fusion Proteins
…els, and a novel virus-spiking strategy, the authors have developed an alternative Fc-fusion protein polishing process that incorporates flow-through strategy in polishing steps using cation exchange…

Article Optimizing Cation-Exchange Chromatography with High-Throughput Process Development for mAb Purification
The typical platform process is generally comprised of an initial capture step with Protein A-chromatography, a first polishing step with cation-exchange media, and a final polishing step using anion-…

Article SEC in the Modern Downstream Purification Process
In general, SEC is considered a low-resolution separation technique, as it does a poor job of discriminating between similar species of molecules and is consequently employed only for the polishing st…

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