Search results for " polishing"
Article
Development of Purification for Challenging Fc-Fusion Proteins
…els, and a novel virus-spiking strategy, the authors have developed an alternative Fc-fusion protein polishing process that incorporates flow-through strategy in polishing steps using cation exchange…
Article
Optimizing Cation-Exchange Chromatography with High-Throughput Process Development for mAb Purification
The typical platform process is generally comprised of an initial capture step with Protein A-chromatography, a first polishing step with cation-exchange media, and a final polishing step using anion-…
Article
SEC in the Modern Downstream Purification Process
In general, SEC is considered a low-resolution separation technique, as it does a poor job of discriminating between similar species of molecules and is consequently employed only for the polishing st…
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Chromatography for the Diversified Antibody Pipeline
It may place more work on the main polishing steps, however, MabSelect may interact with the classes of DH3 in the antibodies—which enables separations targeting the constant DC region. We can see the…
Article
Process Chromatography: Continuous Optimization
He adds that they are also commonly used in polishing steps during mAb purification.
Multimodal resins most often separate molecules based on ligand properties, but there are examples of multimoda…
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Top Tips for Successful Development of Antibody Chromatography Processes
I'm joined today by Jakob Liderfelt, a Global Product Manager for monoclonal antibody polishing with Cytiva. Jakob, thank you so much for your time today.
Jakob Liderfelt: It's very nice …
Article
How to optimize AAV capsid separation
In this free, interactive course you’ll learn how to tackle one of the key challenges in Adeno-associated virus (AAV) manufacturing–separating full and empty capsids in the polishing step. You’ll …
Article
Continuous Manufacturing: A Changing Processing Paradigm
“For downstream polishing steps, the infancy of multi-column chromatography systems is a limitation,” they add.
A change from batch to continuous manufacturing may necessitate new equipment, proce…
Article
Evolution of the Monoclonal Antibody Purification Platform
Use of weak partitioning chromatography
Using weak partitioning chromatography with AEX as the only polishing step has also been proposed as a possible efficient platform (5, 6). Compared to more ty…
Article
Get Started: Purification of mAb Variants - eLearning
- Discover the different strategies for removal of product- and process-related impurities in the polishing steps.
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