Search results for " tangential flow filtration"
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Design Considerations for a Commercial Cell and Gene Therapy Facility
For mAb bioprocessing and viral-vector manufacturing, the equipment needed is similar, such as single-use bioreactors and standard harvest and downstream systems for clarification, tangential flow fil…
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Perfusion Culture Using TFF or ATF as Cell Retention Method
The work demonstrates perfusion culture of an antibody producing Chinese hamster ovary (CHO) cell line, using either tangential flow filtration (TFF) or alternating tangential flow filtration (ATF) as…
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Addressing the Challenges in Downstream Processing Today and Tomorrow
Other processes that are being utilized include tangential flow filtration that takes advantage of the large particle size for separation from smaller cellular contaminants.
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Single-Use for Downstream Chromatography: Benefit or Hindrance?
Single-use technology is gaining ground in downstream bioprocessing, but challenges stall further adoption.
By Feliza Mirasol
ARTMOIS/STOCK.ADOBE.COM
Biopharmaceutical manufactu…
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A Plastic Pipeline for Commercial Bioprocessing?
More than 75% use filled media bags, preassembled tubing sets, depth filters, bioreactors, sampling systems, and tangential flow filtration devices. Less than two-thirds use disposable chromatography …
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Process Chromatography: Continuous Optimization
“Using tangential flow filtration (TFF) between steps reduces product yield and lengthens process times. A salt-tolerant resin can be very beneficial to a purification process by decreasing the need f…
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The New World of Biopharmaceutical Manufacturing
Single-use sensors have been developed for a variety of process parameters for downstream steps such as chromatography, tangential flow filtration, direct filtration, and fill/finish, as well as for s…
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The Challenge of Disruptive Technologies in Bioprocessing
A salt-tolerant resin can be beneficial to a purification process by decreasing the need for dilution or eliminating tangential flow filtration steps all together. In addition, there has been increase…
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Connected Polishing and Concentration Under One Automation Method
Chromatography and tangential flow filtration (TFF) steps are traditionally performed as separate unit operations in downstream bioprocessing. This work aims to demonstrate the capability of the UNICO…
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Extractables Studies for Single‑Use Systems Used in ADC Manufacturing
Single‑use (SU) systems have great potential in antibody‑drug conjugates (ADC) manufacturing. The use of organic solvents in the ADC process might , however, raise questions about potential leac…