Search results for " tangential flow filtration" in Articles / App Notes
Article
Exosome isolation by tangential flow filtration and size exclusion chromatography
The workflows we describe are suitable for scale-up and combine tangential flow filtration (TFF) for the concentration of exosomes followed by gentle size exclusion chromatography (SEC) with our next-…
Article
Challenges in Cell Harvesting Prompt Enhancements
In cell harvesting, depth filters can be used in conjunction with centrifugation or another primary separator, such as tangential flow filtration, as well as being applied directly to whole cell broth…
Article
Updating Viral Clearance for New Biologic Modalities
In terms of traditional viral clearance products, Verlenden continues, studies have shown that concentrating protein solutions using single pass tangential flow filtration (SPTFF) before loading them …
Article
Downstream Processing for Cell-Based Therapies
By Qasim A. Rafiq, Fernanda Masri
With increasing numbers of advanced therapies—including cell and gene therapies—entering early- and late-stage clinical development, significant focus has been …
Article
Achieving Process Balance with Perfusion Bioreactors
Advances in single-use technologies, sensors, and cell retention systems facilitate processes designed for the long run.
By Cynthia A. Challener
MG/Stock.Adobe.com
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Article
Continuous Processing for the Production of Biopharmaceuticals
The authors review the technologies that may help bioprocessing become a truly continuous operation and present case studies that could contribute to the integration of upstream and downstream platf…
Article
Digitalization: The Route to Biopharma 4.0
Industry 4.0, also known as the Fourth Industrial Revolution (4IR), will result in the digital transformation of the manufacturing sector. It is propelled by transformative trends such as the prolif…
Article
Tools for Continuous Bioprocessing Development
For example, perfusion process development experiments (21) using a 4-L working-volume Wave bag and alternating tangential flow (ATF) or tangential flow filtration (TFF) cell-retention filter, consume…
Article
Concentration and Buffer Exchange Using the Automated, Single-Use ÄKTA™ readyflux System
The process was performed according to set specifications for a tangential flow filtration (TFF) process, with the added benefits of the single‑use technology and consistency through automation.
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Article
Downstream Processing Continues to Worry CMOs
A majority of CMOs are considering:
• Use of high capacity resins
• In-line buffer dilution systems
• Single-use disposable tangential flow filtration (TFF) membranes
• Single use-prep…