Search results for " medium" in Articles / App Notes
Article
Scalable ADCF Virus Production Using HyClone™ VaccineXpress Medium
In this study, HyClone VaccineXpress cell culture medium was used for the propagation of influenza virus and rotavirus in an animal-derived component-free (ADCF) process. Small-scale microcarrier cu…
Article
Evaluation of HEK293 Cell Growth and Adenovirus Productivity in HyClone™ CDM4HEK293 Medium
This application note demonstrates growth of HEK293 cells in CDM4HEK293 medium in both shake flask and bioreactor cultures. Adenovirus productivity was evaluated in shake flask cultures. Performance i…
Article
rAAV Production Using HEK293 Cell Line and Transfection Medium
Improving the performance of a scalable AAV process in Xcellerex™ XDR-10 and XDR-200 bioreactor using HyClone™ peak expression medium.
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Article
Evaluation of Hek293 Cell Growth and Adenovirus Productivity in HyClone CDM4HEK293 Medium
This application note demonstrates growth and adenovirus productivity of HEK293 cells and explores the key factors involved in the optimization of cell culture performance and scalability.
Cli…
Article
Concentrating Feed—an Applicable Approach to Improve Antibody Production
Feed-volume reduction can also reduce the cost of consumables, such as disposable bags and medium storage space. Because the feed dilutes the culture and reduces product concentration, the use of lowe…
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Impact of Media Components on CQAs of Monoclonal Antibodies
Arguably the most important and -crucial step in cell culture, however, is the selection of appropriate growth medium for in vitro cultivation. The cell-culture medium provides an artificial environme…
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Implementation of Raw Material Control Strategies in the Manufacture of Single-Use Bioprocessing Containers
Sample preparation was performed by liquid-liquid extraction with dichloromethane at below pH 3, above pH 11, and with no pH adjustment prior to analysis of cell-culture medium incubated in single-use…
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Impact of Manufacturing-Scale Freeze-Thaw Conditions on a mAb Solution
The bags were exposed from 1 to 3 cycles (1x-3x) of either fast freeze/fast thaw, fast freeze/slow thaw, medium freeze/medium thaw, slow freeze/fast thaw, or slow freeze/slow thaw. A liquid control wa…
Article
Optimization of Protein Expression in Escherichia Coli
There are many parameters that affect cell growth and recombinant protein expression, such as choice of medium, carbon source, temperature, pH, aeration, inducer concentrations, and length of inductio…
Article
A Platform Approach for the Identity Testing of Multi-Component Cell-Culture Media
A combined approach of NIR and MVDA was used for the identification and qualification of basal medium powder components (9) and was evaluated as an identity tool for cell-culture media (10).
Raman…