Search results for " tag"

Article Purifying Tagged Proteins: Learn the Basics
When and why should you use it? Learn the basics in our quick guide to tagged protein purification.

Article Affinity chromatography Handbook: Vol. 2 Tagged Proteins
The advantages of using a protein/peptide tag fused to the recombinant protein to facilitate its purification and detection are now widely recognized. In some cases, tags may improve the stability and…

Article Generating a Fully Processed Antibody
…GE cell line to produce cell lines and processes capable of expressing and converting high titers of tagged antibody. The FGE cell line needed to have all the normal phenotypic traits of the base CHO…

Article Optimization of Protein Expression in Escherichia Coli
Affinity tagging The addition of affinity tags may aid the detection and purification of target proteins. Common affinity tags include poly-His, FLAG, c-Myc, poly-Arg, and StrepII tags. The tag cou…

Article Bioterminology: A Guide to the Biopharmaceutical Lexicon
Bioterminology: A Guide to the Biopharmaceutical Lexicon This lexicon was generated by the editors of BioPharm International magazine. Please feel free to email your suggestions for terms that s…

Article Your Protein Purification Questions Discussed
In one of GE´s web chats, ÄKTA™ system users asked questions relating to lab-scale protein purification and ÄKTA systems, which experts answered during a live session. The expert pan…

Article Process Chromatography Selection for Downstream Processing Applications
By contrast, immobilized metal affinity chromatography is not often used because tagged proteins are uncommon in process scale. Becerra-Arteaga (MilliporeSigma): Ion-exchange chromatography is mos…

Article Affinity Chromatography Handbook in 3 Volumes
2: Tagged Proteins Affinity Chromatography, Vol. 3: Specific Groups of Biomolecules Download them all here

Article Analysis of Glycosylation in Biosimilars
One such technique uses fluorescent tagging of released native glycans with 2-Aminobenzamide (2-AB) followed by hydrophilic interaction chromatography (HILIC). Figure 5 shows 2-AB stacked chromatogram…

Article Affinity Chromatography Handbook: Vol. 1 Antibodies
The use of recombinant technology opens up the potential to create an infinite number of combinations between immunoglobulins, immunoglobulin fragments, tags and selected proteins, further manipulatin…

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