Search results for " tag"
Article
Enhancing Resins Addresses Purification Concerns
Another possible game changer, he notes, would be if a universal tag system were developed and approved for large-scale downstream processing, and not only for small scale or academic applications, wh…
Article
Track-and-Trace Progress Benefits Supply-Chain Security Efforts
The company decided to serialize its Diprovan anesthetic, a workhorse generic product, using RFID tags containing the four identifiers (2).
Although counterfeiting remains a threat, the growing us…
Article
Your Protein Purification Questions Discussed
In one of Cytiva´s web chats, ÄKTA™ system users asked questions relating to lab-scale protein purification and ÄKTA systems, which experts answered during a live session.
The expert…
Article
Process Chromatography Selection for Downstream Processing Applications
By contrast, immobilized metal affinity chromatography is not often used because tagged proteins are uncommon in process scale.
Becerra-Arteaga (MilliporeSigma): Ion-exchange chromatography is mos…
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Affinity Chromatography Handbook in 3 Volumes
2: Tagged Proteins
Affinity Chromatography, Vol. 3: Specific Groups of Biomolecules
Article
Analysis of Glycosylation in Biosimilars
One such technique uses fluorescent tagging of released native glycans with 2-Aminobenzamide (2-AB) followed by hydrophilic interaction chromatography (HILIC). Figure 5 shows 2-AB stacked chromatogram…
Article
Affinity Chromatography Handbook: Vol. 1 Antibodies
The use of recombinant technology opens up the potential to create an infinite number of combinations between immunoglobulins, immunoglobulin fragments, tags and selected proteins, further manipulatin…
Article
Antibody Purification Handbook
The use of recombinant technology opens up the potential to create an infinite number of combinations between immunoglobulins, immunoglobulin fragments, tags and selected proteins, further manipulatin…
Article
Implications of Cell Culture Conditions on Protein Glycosylation
In an alternative approach, a fluorescence tag—2-aminobenzamide—can be used to specifically label the released N-glycans and these labeled N-glycans can then be chromatographically separated. The chro…
Article
Advanced Technologies Facilitate Scale-up and Technology Transfer
Single-use and modular technologies plus continuous manufacturing are increasingly important to biopharma scale-up and tech transfer.
Jun 01, 2015
By Cynthia A. Challener
BioPharm Inte…