Search results for "N-1"
Article
Perfusion N-1 – A Way to Improve the Fed-Batch Production Process
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In this article, we show how the conventional N-1 seed train step was replaced by a high-density perfusion and how the production bioreactor can be seeded at a 10-fol…
Article
Improving Upstream Predictability
Used with high cell density cryopreservation, perfusion in N-1 and N-2 bioreactors can reduce the cost of goods by 13.5%, shortening run times and increasing production bioreactor titers, he says.
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Article
Intensifying your fed-batch process using cryobags
We will show that high-density cryobags can be used to seed a bioreactor at a 500-fold higher seed density, reducing the total process time by up to 9 days.
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Article
Analytical Considerations for Successful Upstream Process Development with Spivey and Lane
Others are just growing the cells in what's called an N-1 fashion. That means you're going to use those cells about three or four days later to inoculate a larger bioreactor. The use case there is tha…
Article
Maximum Output Starts with Optimized Upstream Processing
Samsung implemented large-scale N-1 perfusion to enable inoculation of production bioreactors (15,000 L) at higher cell densities and achieve peak cell densities within shorter culture durations. The …
Article
Implementation of Process Intensification Strategies Across the Entire Cell Culture Workflow
Andreas Castan, Strategic Technology Collaborations Leader at Cytiva, will discuss the latest trends across the industry in relation to process intensification and give specific examples of how both N…
Article
Achieving Process Balance with Perfusion Bioreactors
Additionally, Pall Biotech has seen growing interest in N-1 perfusion bioreactors to increase the seed density of the N bioreactor (which is often fed-batch). The goal, according to Levison, is to int…