Search results for "PCR"
Article
A Risk-Based Genetic Characterization Strategy for Recombinant CHO Cell Lines Used for Clinical and Commercial Applications
FHD=first human dose, FRD=first registration dose, PCR=polymerase chain reaction, qPCR=quantitative polymerase chain reaction, MCB=master cell bank, RT-PCR=reverse transcription polymerase chain react…
Article
Emerging Therapies Test Existing Bioanalytical Methods
Specialty assays used to understand the distribution, function, efficacy, and immunogenicity of gene- and cell-therapy drugs—including cell-based neutralizing assays, qPCR, flow cytometry and enzyme-l…
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Best Practices for Selecting a Top-Quality Cell Line
To detect low levels of CAR genes and microbial contaminants and precisely quantify CAR genes, Bio-Rad’s Droplet Digital PCR (ddPCR) technology is of increasing interest. The technology selectively am…
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Concentrating Feed—an Applicable Approach to Improve Antibody Production
The improvement of productivities was likely due to the enhancement of protein folding and secretion machinery suggested by quantitative polymerase chain reaction (qPCR) array assay. Supplementing sur…
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Ensuring the Biological Integrity of Raw Materials
…niversity of California San Francisco, tested eight viral vaccines using polymerase chain reaction (PCR) and DNA sequencing, and found that three of the vaccines contained unexpected viral sequences.…
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Application of Quality by Design to Viral Safety
Massively parallel sequencing or other new technologies such as PCR with mass spectrometry or virus microarrays can be used to identify a panel of potential contaminants. For instance, massively paral…
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Alternative solutions to separate AAV full and empty capsids using anion exchange
And separation results were confirmed by PCR, ELISA, mass photometry, and analytical ultracentrifugation. We show that these AEX approaches are reproducible and can be scaled up.
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COVID-19 Diagnostics Supplier Ramps Up Production with Help of Cytiva’s Testa Center in Sweden
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In this blog post, you can learn about how Solis BioDyne, Estonian manufacturer of polymerase chain reaction/quantitative polymerase chain reaction reagents to analyze DNA f…
Article
Ensuring Viral Safety of Viral Vaccines and Vectors
Cell lines must also be tested for species-specific viruses, as appropriate, using antibody tests or polymerase chain reaction (PCR) panels.
Figure 3. Test strategy for ensuring cell banks and …
Poster
Residual DNA analysis in influenza vaccine processing
To reliably measure residual DNA in both process samples and final vaccine by quantitative PCR (qPCR), DNA preparation prior to analysis is a necessity. Samples from the vaccine purification process c…