Search results for "cation-exchange"
Article
Optimizing Cation-Exchange Chromatography with High-Throughput Process Development for mAb Purification
The typical platform process is generally comprised of an initial capture step with Protein A-chromatography, a first polishing step with cation-exchange media, and a final polishing step using anion-…
Article
Improving Process-Scale Chromatography
Pall recently introduced a cation-exchange mixed mode sorbent that is effective in post-Protein A mAb polishing when used in conjunction with membrane chromatography in the flow-through mode.
Cyti…
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Process Chromatography Selection for Downstream Processing Applications
For example, acetate is commonly used as an elution buffer on Protein A capture as well as a bind-and-elute buffer in the cation-exchange step of a mAb process. Additional factors include cost, ease o…
Article
Evolution of the Monoclonal Antibody Purification Platform
Various authors have described cation-exchange chromatography as a capture step in mAb purification (8-11). Though it may not be as efficient as Protein A in terms of selectivity of HCP, it can be use…
Article
HT Multi-Product Liquid Chromatography for Characterization of Monoclonal Antibodies
Quantitative analysis of monoclonal antibodies by cation-exchange chromatofocusing. J Chromatogr A. 2009;1216:5989–599.
27. Farnan D, Moreno GT, Stults J, Becker A, Tremintin G, van Gils M. Interl…