Often, an ion exchange unit operation will involve high loadings of sample and step-wise elution to wash out some impurities and then recover the product separated from other unwanted components. However, it is always wise to have studied the separation at low sample loading with a smooth, shallow gradient. Although steps are great to maximize sample loading while still separating product from impurities, they may give peaks in the chomatogram that can be misinterpreted as distinct components. The shallow-gradient/low sample load run gives a good indication of the components that can be resolved. If you are interested in learning more, you might want to attend a Fast TrakTM course. Follow this link to see the full training program and nearest scheduled courses.
December 13, 2011
Avoid misunderstanding an elution profile