June 2, 2022

Accelerating VLP purification process development

See how using mechanistic modeling can speed up the purification process development of virus-like particles (VLPs).

Indeed, a method for the large-scale production of VLPs is production in suspension cell culture, and extracting the VLPs from the cell culture supernatant is a time and labor-intensive process.

The development of a purification strategy is a mainly experimental approach where parameters are optimized via Design-of-Experiment (DoE) studies. Dozens of experiments are needed to screen a process design space that was manually chosen.

In contrast, mechanistic models describe the transport of sample components using fluid dynamic and thermodynamic principles. Further, they allow for efficient process optimization in silico.


Tags: downstream, mechanistic modeling, chromatography, VLP, process development