Buffer preparation is both time-and space-consuming and can easily become a challenge in biomanufacturing. This application note describes a lean approach to buffer preparation by implementing in-line conditioning (IC). Buffers of different formulations for a mAb chromatography capture step were prepared in an automated, consecutive manner, minimizing manual interactions. To further reduce the time and space required, ready-made stock solutions delivered in disposable bags were connected directly to the Inline Conditioning system and feedback regulation of the final buffers, using dynamic control, ensured accurate formulations.
August 10, 2017