May 4, 2023

Developing a HIC polishing step for removal of mAb aggregates

This article describes the development of a flow-through (FT) polishing step using hydrophobic interaction chromatography (HIC) to remove mAb aggregates.

It includes:
- The use of HTPD plates to select the optimal resin as well as running conditions
- The use of a design of experiments (DoE) to optimize the running conditions
- A Monte Carlo simulation to identify the robust conditions to reach the objectives in aggregation removal and the monomer recovery.

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Tags: Process development; chromatography; protein purification; downstream; mAb; antibody