September 8, 2023

Fundamentals of hydrophobic interaction chromatography

Hydrophobic interaction chromatography (HIC) is a versatile technique that can be used for capture, intermediate purification, or polishing steps.

This technique separates proteins according to differences in their surface hydrophobicity, and is suitable for the purification of a large range of molecules: 
* recombinant proteins and peptides 
* monoclonal antibodies (mAbs), bispecific mAbs and antibody-drug conjugates (ADC)
* mRNA
* plasmid 

New to HIC? Browse this HIC resource library, containing our recently updated handbook, application notes and more!

Tags: HIC; chromatography; process development; Protein purification; mAb purification; hydrophobic interaction chromatography