February 9, 2024

Get Started: Purification of mAb Variants - eLearning

The success of monoclonal antibodies (mAbs) as therapeutics and the multi-domain structure of antibodies has made it possible to create antibody variants, such as bispecific antibodies (bsAbs) and smaller antibody fragments.

Purification of bsAbs and antibody fragments isn’t as straightforward as for mAbs. These molecules show wider variation in binding affinities and are often more challenging to separate from impurities, such as host cell proteins (HCP), host DNA, aggregates, and isoforms.

In this free, interactive course you’ll learn which purification strategies overcome these challenges. You’ll also find useful tips for different chromatography steps.

Key learnings:

- Learn the important considerations for selecting a purification protocol.
- Understand which resins are suitable for the first capture step.
- Discover the different strategies for removal of product- and process-related impurities in the polishing steps.


Tags: Antibody variants, bispecific antibody, antibody fragment, VHH, scFv, Fab, chromatography, resin, affinity, Protein A, polishing, IEX, ion exchange