September 27, 2018

How to Determine Dynamic Binding Capacity (DBC) of Chromatography Resins

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In protein purification, dynamic binding capacity (DBC) of a chromatography column describes the maximum amount of target protein that you can load onto your column without causing unnecessary loss, measured under realistic experimental conditions (default flow rate, real protein sample). In some cases, you might need to determine the DBC yourself to screen for the best resin and running conditions for your specific protein.  

Tags: column, resin, DBC, dynamic binding capacity, chromatography, protein purification