April 8, 2021

Sanitization Opportunities for Protein A Resins

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Bacterial endospores present a challenge in bioproduction due to their resistance to common sanitization procedures using high concentrations of NaOH. Several protein-based chromatography resins are also sensitive to such harsh sanitization conditions. As an alternative, the oxidizing agent peracetic acid (PAA) could be used in the control of such contaminants.

Here, we describe an approach to safely sanitize our new generation bioprocess protein A affinity resin for antibody purification—MabSelect PrismA—with 30 mM PAA combined with NaOH cleaning-in-place regimen in a resin lifetime study.

Tags: chromatography, sanitization, Protein A resin, MabSelect PrismA, bioburden