February 14, 2019

Single-Step, Protein A Chromatography Process for Bispecific Antibodies in Early Screening

A single-step downstream process using protein A chromatography to purify a bispecific antibody (BsAb) in early screening has been successfully developed to replace an existing three-step process. The goal was to separate BsAb monomer from fragments such as the individual heavy and light chain pairs while achieving high purity and yield of BsAb. MabSelect™ PrismA protein A resin was selected for the capture step. Most fragments could be removed as early as the capture step using gradient elution from pH 5 to 3.5, without the need for additional purification steps. BsAb constructs were efficiently purified to give more than 90% purity and 85% yield in the early screening downstream process.

Learn more about bispecific purification

Tags: monoclonal antibodies, Mab, chromatography, Protein A resin, bispecific antibodies, bispecific