In the first of a two-part series, Anki Magnusson, Staff Research Engineer, Protein and Viral Production, and Åsa Hagner-McWhirter, PhD, Senior Scientist, Bioprocess Applications R&D, both at Cytiva, explore obstacles faced during adeno-associated virus (AAV) process development from both the upstream and downstream perspective.
Topics covered include:
1:00 – Two main challenges for upstream AAV process development, including which development platform to use and scalability
3:09 – Parameters evaluated for AAV plasmid transfection
4:27 – Three main challenges for downstream AAV process development, including cell lysis and the polishing step for full capsid enrichment
7:28 – Challenges associated with full and empty capsid analytics
Learn more about AAV production:
Webinar on demand: Start-to-finish AAV production