Search results for " DNA" in Articles / App Notes
Article
A Risk-Based Genetic Characterization Strategy for Recombinant CHO Cell Lines Used for Clinical and Commercial Applications
After the sequence of the expression plasmid is confirmed, the expression plasmid DNA is usually isolated at larger scale to prepare sufficient plasmid DNA to enable cell-line generation. From time to…
Article
Virus-like Particles as Therapeutic Moieties of the Future
VLPs have been shown to encapsulate a variety of cargo including foreign DNA, plasmid DNA, RNA (4), genome editing machinery, siRNA, proteins, and peptides for in-vitroand in-vivo applications. Furthe…
Article
A Look at the Affinity Chromatography Landscape
…ypically served as a method for removal of process-related impurities such as host-cell proteins and DNA, with Protein A the most widely used resin. Resin suppliers have in recent years been challeng…
Article
Enhancing Resins Addresses Purification Concerns
Deorkar also explains that plasmid DNA (pDNA) and recombinant adeno-associated viruses (rAAvs) are two other classes of biologic molecules that are of high interest for the biopharmaceutical industry …
Article
The Development and Application of a Monoclonal Antibody Purification Platform
…ducts, processing variants) and cell-derived impurities such as host cell protein (HCP) and cellular DNA. In response, platform purification processes for MAbs, i.e., those designed to move a product…
Article
Gene Therapies Push Viral Vector Production
• AAV exhibits reduced risk of ectopic integration of the therapeutic DNA.
“Adenovirus (AV) was the first viral vector investigated for gene therapy use; however, after the death of a pati…
Article
Can Vaccine Development Be Safely Accelerated?
As an example, he points to the development of subunit vaccines that rely on recombinant DNA to encode a critical subunit of the vaccine that generates a response. There are additional challenges to t…
Article
Re-use of Protein A Resin: Fouling and Economics
Specificity of Protein A ligand towards mAb results in extremely effective clearance of host cell proteins (HCP), viruses, and DNA (2–5). The high selectivity is accompanied by reasonably good physioc…
Article
Evolution of the Monoclonal Antibody Purification Platform
Typical steps include:
• Protein A chromatography for capture of the product and removal of host cell-related impurities (host cell proteins and DNA). Though extremely efficient and effective,…
Article
Choose your Optimal Wavelength for Chromatography
U9-T monitor allows differentiation between peaks containing protein and those containing DNA/RNA, nucleic acids or nucleotides. Most proteins absorb strongly at 280 nm due to the presence of tryptoph…