Search results for "column"
Article
Efficient Sodium Hydroxide Sanitization with a Novel Procedure of AxiChrom™ Columns Packed with Chromatography Resin
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Sodium hydroxide (NaOH) cleaning-in-place is the widely accepted method of sanitization employed by biopharmaceutical manufacturers to avoid the excessive costs and delays of a…
Article
New Selection Guide: Ion Exchange Chromatography Columns and Resins
Use these decision trees to find the most appropriate resin for your needs.
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Article
Optimizing Resin Performance with Disposable Chromatography Solutions
The experts addressed how disposable chromatography columns can be used throughout the life of a campaign, the costs and benefits associated with using prepacked columns, the complexities of resin str…
Article
Process Chromatography Selection for Downstream Processing Applications
Important factors such as column design, resin development, and automation have all played a role in simplifying the purification process, in some cases reducing it from five steps to three (1). Selec…
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Making the Move to Continuous Chromatography
Switching from batch to continuous allows for improved resin capacity utilization through continuous loading (overloading, in fact) of the column or membrane for increased process efficiency. It is pa…
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Re-use of Protein A Resin: Fouling and Economics
A typical Protein A chromatography process consists of five steps: pre-equilibration and equilibration of the column; capture of the mAbs from the harvest; wash step with the selective buffer to remov…
Article
SEC in the Modern Downstream Purification Process
The hydrodynamic properties of the molecules in the sample may be determined, for example, by comparing the chromatogram of the sample to be purified on a high-performance liquid chromatography column…
Article
Mechanistic Modeling of Preparative Ion-Exchange Chromatography
Mass-transfer models
The transport of solute through the various phases inside the packed bed column has been described using mathematical models, albeit with different level of details (1). Mecha…
Article
Addressing the Challenges in Downstream Processing Today and Tomorrow
Weak partitioning chromatography (WPC) and a high throughput screening (HTS) tools have been essential for development of a robust two-column platform purification process. The benefits of a two-colum…
Article
Continuous Chromatography: Trends, Definitions and Approaches
All of the acronyms describe the same thing: a chromatography capture step-based utilizing multiple columns operated in a merry-go-round manner, where columns moved against the direction of feed flow.…