Search results for " fed-batch" in Articles / App Notes
Article
Optimization of Fed‑Batch Culture Conditions for a mAb‑Producing CHO Cell Line
The selected Cell Boost supplements were thereafter applied in fed-batch cultures to fine-tune their relative ratios. The selected fed-batch culture conditions supported mAb titers of approximately 4 …
Article
Continuous Manufacturing: A Changing Processing Paradigm
…ge portfolio of short-run manufacturing products, and “scaling process development is easier using fed-batch technologies” (1). Langer also writes that, based on data from his yearly report, “there h…
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Achieving Process Balance with Perfusion Bioreactors
As with batch and fed-batch processes, single-use bioreactors offer numerous other advantages over stainless steel. “Users can eliminate most of the cleaning and validation steps that are associated w…
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Trends in Single-use Bioreactors
Another example of how single-use systems can facilitate continuous processing is the ease with which a single-use bioreactor that has been operated in a fed-batch manufacturing process can be readily…
Article
Digitalization: The Route to Biopharma 4.0
Glycosylation Flux Analysis Reveals Dynamic Changes of Intracellular Glycosylation Flux Distribution in Chinese Hamster Ovary Fed-Batch Cultures. Metab. Eng. 2017, 43, 9–20. DOI: 10.1016/j.ymben.2017.…
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Biomanufacturing: Demand for Continuous Bioprocessing Increasing
Negative assessments from within the industry of continuous perfusion fed-batch processing overall may reflect a lack of direct exposure or experience with continuous technology.
In BioPlan’s a…
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Maximizing the Effectiveness of Upstream Bioprocessing Through the Combination of Lab Informatics and Instrumentation
…roductivity with their WuXiUP continuous perfusion-based processing platform compared to traditional fed-batch, which enables 1000-L single-use bioreactors to produce the same amount as 10,000-L stai…
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Cell-Culture Advances Test Bioreactor Performance Models
He points to the higher cell densities in perfusion mode, which result in increased oxygen demand in a higher viscosity solution with potentially more cell-free biopolymers and lipids than a fed-batch…
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Scaling Up Novel Therapies
One way they do this, he says, is by “scaling out,” intensifying processes, for example by using fed-batch and perfusion on the upstream side, and multicolumn operations, whether it’s just higher cycl…
Article
Impact of Media Components on CQAs of Monoclonal Antibodies
CHO cells in fed-batch mode at low glutamine (17% (w/w) hampered the rate of deamidation up to 40%. Polyols at high concentrations displayed a protective effect on oxidation. Addition of 0.01% (v/v) P…