Search results for " hydrophobic interaction"
Article
Optimization of a HIC step for recombinant protein purification
Follow this step-by-step high-throughput process development (HTPD) workflow to determine the optimal resin and initial conditions for hydrophobic interaction chromatography (HIC).
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Article
Addressing the Complex Nature of Downstream Processing with QbD
“Polishing steps such as ion exchange, hydroxyapatite, and hydrophobic interaction chromatography can be more heavily influenced by pH, conductivity, loading capacity, residence time, and temperature,…
Article
A Q&A With Prof. Alois Jungbauer: The Role of HIC in Biotech
Process Development Forum speaks with Professor Alois Jungbauer, Department of Biotechnology, BOKU, Vienna, about Hydrophobic Interaction Chromatography (HIC).
What is your view of HIC in the bi…
Article
Analyzing Proteins Using SEC, MALS, and UHPLC
Light scattering analysis combined with more rapid size exclusion chromatography improves protein characterization.
The characterization of proteins involves identification of their complex …
Article
Addressing the Challenges in Downstream Processing Today and Tomorrow
This unwanted modification required an extra process step using hydrophobic interaction chromatography (HIC) to separate these modified molecules from the desired product. E. coli expression often yie…
Article
The Challenge of Disruptive Technologies in Bioprocessing
Thermo Fisher Scientific aims to address this challenge with a series of commercialized hydrophobic interaction chromatography resins with a differentiating range of hydrophobicity, resolution, high c…
Article
Developing a HIC polishing step for removal of mAb aggregates
This article describes the development of a flow-through (FT) polishing step using hydrophobic interaction chromatography (HIC) to remove mAb aggregates.
It includes:
- The use of HTPD plate…
Webcast
Optimizing a HIC step with HTPD
This webinar discusses a step-by-step workflow for optimizing a hydrophobic interaction chromatography (HIC) step.
Article
Webinar: Optimizing a HIC step with HTPD
Webinar date: Wed, Mar 8, 2023 11:00 AM EST (5:00 PM CET)
This webinar discusses a step-by-step workflow for optimizing a hydrophobic interaction chromatography (HIC) step.
Thi…
Article
Adsorption isotherm models for chromatography
In this article we will discuss adsorption models for ion exchange, hydrophobic interaction, and mixed mode chromatography.
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