Search results for " binding"
Article
Ligand-Binding Assays and the Determination of Biosimilarity
The importance of binding activity
Most biotherapeutics rely on specific binding activities to achieve a clinical effect. Therefore, matching the binding activities of a biosimilar candidate to th…
Article
A Closer Look at Affinity Ligands
Affinity chromatography is a separation method based on the specific binding interaction between an immobilized affinity ligand and its binding partner. The most common example in the realm of bioproc…
Article
Optimizing Cation-Exchange Chromatography with High-Throughput Process Development for mAb Purification
This step often requires more optimization than initial capture and/or final polishing and aims to achieve high-dynamic binding capacity and maximum aggregate and host-cell protein (HCP) clearance. In…
Article
A Look at the Affinity Chromatography Landscape
Specific binding interactions between biologic molecules and immobilized affinity ligands allow for selective separations. With the advent of next-generation biopharmaceuticals, chromatography materia…
Article
Single-Domain Antibodies for Brain Targeting
Favorable properties, such as good expression, thermal stability, and solubility, are co-selected with binding activity using phage libraries, such as in the case of human VH domain antibodies (21), a…
Article
Evolution of the Monoclonal Antibody Purification Platform
Compared to more typical flow-through chromatography, here the conductivity and pH are chosen such that the binding of both the product and impurities are enhanced, attaining an antibody partition coe…
Article
Process Chromatography: Continuous Optimization
Suppliers of chromatography resins are developing new resin chemistries and binding mechanisms to meet this need.
Several interaction modes
There are four major chromatographic techniques employed…
Article
Mechanistic Modeling of Preparative Ion-Exchange Chromatography
Development of a mechanistic model requires three major steps: modeling of mass transfer, modeling of binding, and finally, validation of the mechanistic model. The choice of details depends on the de…
Article
Re-use of Protein A Resin: Fouling and Economics
It has been shown that the extended use of a resin results in decrease in its binding capacity, a decrease in product recovery, and a loss of Protein A ligands (9). Recent innovations in Protein A-aff…
Article
Development of Purification for Challenging Fc-Fusion Proteins
Anion exchange chromatography (AEX) (6-8, 10-11), typically serves as a polishing chromatographic step operated in flow-through mode, binding process-related impurities such as HCP, DNA, leached ProA,…