Search results for " affinity" in Articles / App Notes
Article
Enhancing Resins Addresses Purification Concerns
These tails reversibly bind to a poly(T) ligand that can be immobilized to a solid support, which would enable an affinity-type of purification. “This is why oligo(dT) support is often used in the pur…
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The Challenge of Disruptive Technologies in Bioprocessing
An example of the latter may be the use of affinity chromatography in the capture step of antibody processes, Jagschies says.
“When we look at the biologics market dynamics for treatment of dis…
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Improving Process-Scale Chromatography
She points to several new types of affinity chromatography resins introduced to the market in recent years that target recombinant plasma proteins, gene therapy applications, and different types of an…
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Optimization of Protein Expression in Escherichia Coli
Affinity tagging
The addition of affinity tags may aid the detection and purification of target proteins. Common affinity tags include poly-His, FLAG, c-Myc, poly-Arg, and StrepII tags. The tag cou…
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Comparing Protein A Resins for Monoclonal Antibody Purification
Protein A is the affinity chromatography ligand of choice for the first antibody capture step, because its high selectivity gives excellent purity (typically > 99%) and high yields. Furthermore, Prote…
Article
FAQs about Cytiva™ Protein Select™ technology
One chromatography step does the affinity purification, a traceless tag cleavage, and the tag removal. The pure protein obtained is in its native state with no traces of the tag amino acids. The proto…
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Video: Standardize recombinant protein purification process development
During an affinity step performed with Cytiva Protein Select resin, the protein self-cleaves from the tag and elutes with no residual tag amino acids.
This technology standardizes purification duri…
Article
As the therapeutic pipeline diversifies, chromatography toolboxes are expanding.
Cytiva expands the antibody capture resin toolbox and has recently announced MabSelect™ VH3 affinity resin. MabSelect VH3 uses an engineered protein A resin that interacts only with the variable heavy…
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Next Generation Process Chromatography
Binding capacities, range of selectivity, and the stability of affinity ligands have been vastly improved. One of the main remaining challenges is that each purification cycle today typically takes >3…
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Preclinical Evaluation of Product Related Impurities and Variants
First, the binding affinity of GCSF variant to GCSF-R followed the order: Reduced> Pure=Oxidized=f-Met> Aggregate. The enhanced binding affinity of the reduced GCSF samples was attributed to stabiliza…