Search results for "ion-exchange chromatography"
Article
SEC in the Modern Downstream Purification Process
Biomolecules typically should not bind to an SEC resin like they do in ion-exchange chromatography, affinity chromatography, hydrophobic interaction, or mixed-mode chromatography. As a result, mobile-…
Article
Downstream Processing Continues to Worry CMOs
CMOs are working hard to improve performance by investigating new technologies for filtration and purification.
By Eric S. Langer
Downstream processing continues to be a significant pain poi…
Article
Impurity Testing of Biologic Drug Products
Experts share insights on the various methods used for purity and impurity analysis of therapeutic proteins.
By Adeline Siew, PhD
CA-SSIS/shutterstock.comIm…
Article
Challenges of Protein Aggregation During Purification
Ion-exchange chromatography, on the other hand, exploits differences in charges and dipole moments to separate products from impurities. In particular, protein aggregates are often separated from the …
Article
QbD and PAT in Upstream and Downstream Processing
To gain perspective on the implementation of quality by design (QbD) and process analytical technology (PAT) in biopharmaceutical processing, BioPharm International spoke with Clinton Weber, as…
Article
2014 Manufacturing Trends and Outlook
Ion-exchange chromatography was the most frequently reported (71.6%) followed by chromatography with other resins (62.7%), chromatography with Protein A (56.7%), and membrane-based filters (53.7%).
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Article
Mechanistic Modeling: Does it Have a Future in Process Development?
For some unit operations, such as ion-exchange chromatography, mechanistic models are easily employed for prediction. However, when it comes to reverse-phase chromatography or HIC, the mathematical de…
Article
Quality by design for biotechnology products—part 1
Examples include:
analytical tools such as native isoelectric focusing (IEF), ion-exchange chromatography (IEC), and online mass spectrometry to quantify the level of deamidation
preclin…
Article
Host-Cell Protein Measurement and Control
Jun 01, 2015
By Fengqiang Wang, PhD, Daisy Richardson, Mohammed Shameem
BioPharm International
Volume 28, Issue 6, pg 32–38
Host-cell proteins (HCPs) constitute a major part of proce…