Search results for " recombinant protein" in Articles / App Notes
Article
A Risk-Based Genetic Characterization Strategy for Recombinant CHO Cell Lines Used for Clinical and Commercial Applications
Over the past several decades, numerous recombinant proteins have been approved as therapeutic drugs by regulatory authorities, and many more are currently undergoing clinical development (1). Chinese…
Article
Elucidating Biosimilars Characterization
NIBRT: Differences regarding the approval process for small molecules as compared to recombinant protein therapeutics are reflected in the complexity that must be considered when comparing small-molec…
Article
A Closer Look at Affinity Ligands
Affinity ligands play an exciting role in the ever-growing sphere of downstream processing. In speaking with BioPharm International®, Phillip Elliott, PhD, associate director of Process Development,…
Article
Generating a Fully Processed Antibody
Protein conversion by FGE
Figure 1: Formylglycine-generating enzyme (FGE) protein recognition site and conversion process in the context of a recombinant protein of interest.
Formylglycine-gene…
Article
Best Practices for Selecting a Top-Quality Cell Line
… organization of chromatin in all mammalian cells and allow for higher and more stable expression of recombinant proteins.
A panel of tens to hundreds of high-expressing clones are assessed fo…
Article
Fermentation for the Future
coli, is the formation of insoluble aggregates of the overexpressed recombinant protein, referred to as IBs. In most cases, the IBs consist largely of the protein of interest, but may also include oth…
Article
Quality by design for biotechnology products—part 1
A PhRMA Working Group's advice on applying QbD to biotech.
By Taruna Arora, Roger Greene, Jennifer Mercer, Paul Tsang, Meg Casais, Stuart Feldman, Jutta Look, Tony Lubiniecki, Joseph Mezzatesta,…
Article
HT Multi-Product Liquid Chromatography for Characterization of Monoclonal Antibodies
Application of imaging capillary IEF for characterization and quantitative analysis of recombinant protein charge heterogeneity. Electrophoresis. 2008;29:4368–76.
23. Vasilyeva E, Woodard J, Taylo…
Article
A Look at the Affinity Chromatography Landscape
“Engineered recombinant Protein A resins with smaller bead sizes are often suitable for bispecific antibodies with Fc regions, while for bispecific molecules without Fc regions, other affinity resins …
Article
Improving Process-Scale Chromatography
Advances in technology are increasing the productivity and efficiency of commercial-scale chromatography bioprocesses.
By Cynthia Challener, PhD
Chromatography is a crucial step in the purif…