Search results for " separation"
Article
Updating Viral Clearance for New Biologic Modalities
Potential viral separation challenges center around the diversity or variety of potential viral contaminants, he says. First, dependent on the production cell line, there may be a number of viruses wi…
Article
Using Quality by Design to Develop Robust Chromatographic Methods
A QbD approach to understanding a measurement system such as a chromatographic method involves more than the demonstration of a depth of understanding regarding the choice of chromatographic separatio…
Article
Optimizing Resin Performance with Disposable Chromatography Solutions
The Importance of resin structure
BioPharm: How important is resin structure to separation success?
Royce and Lundström (Cytiva): Resin structure is crucial to separation success, and there ar…
Article
Enhancing Resins Addresses Purification Concerns
“There are several purification techniques that are in use, such as precipitation, tangential flow filtration, or chromatographic separations. However, precipitation or size-exclusion chromatography (…
Article
Challenges in Cell Harvesting Prompt Enhancements
Cell harvesting involves the separation of the cell-culture from the medium and running it through several techniques: centrifugation, microfiltration, depth filtration, and filtration through absolut…
Article
Implications of Cell Culture Conditions on Protein Glycosylation
Characterization using mass spectrometry
The aforementioned permethylation procedure allows the separation of glycans with similar masses in the non-derivatized state. For example, two glycans—with…
Article
Analyzing Proteins Using SEC, MALS, and UHPLC
Better separation always results in a better characterization,” he asserts.
Koza and Birdsall also note that SEC is rather insensitive to small changes in apparent MW, and non-specific interaction…
Article
A Closer Look at Affinity Ligands
Affinity chromatography is a separation method based on the specific binding interaction between an immobilized affinity ligand and its binding partner. The most common example in the realm of bioproc…
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Top Tips for Successful Development of Antibody Chromatography Processes
Then, he shares his top tips for addressing challenges associated with mAb aggregates removal and separation of homo and heterodimers.
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Article
Technology Innovations Improve Process Chromatography Performance
By Feliza Mirasol
Selecting an appropriate separation technology for biologic manufacturing is a crucial task in maximizing productivity in downstream bioprocessing. Biomanufacturers consider se…