Search results for "host"
Article
Addressing the Challenges in Downstream Processing Today and Tomorrow
Early biotechnology products in the US and Europe were characterized by low titers and low cell densities using various cell hosts (1, 2). Most companies had few recombinant products licensed or in de…
Article
A Risk-Based Genetic Characterization Strategy for Recombinant CHO Cell Lines Used for Clinical and Commercial Applications
Chinese hamster ovary (CHO)-derived cell lines have become the predominant host for the manufacturing of glycosylated therapeutic proteins. During this period of explosion in the application of CHO ex…
Article
Virus-like Particles as Therapeutic Moieties of the Future
Besides these applications in therapeutics, VLPs are an excellent model system for studying the initial host interaction pathways of biosafety level 4 restricted viruses and the modes of virus assembl…
Article
Combining Microbioreactors and Advanced Statistical Techniques to Optimize a Platform Process for a New Host-Cell Line
…ple (using artificial data) of the optimization of an existing platform process to better fit a new host-cell line. When a new host-cell line is introduced, the starting point for the process will us…
Article
Evolution of the Monoclonal Antibody Purification Platform
Typical steps include:
• Protein A chromatography for capture of the product and removal of host cell-related impurities (host cell proteins and DNA). Though extremely efficient and effective,…
Article
Reimagining Affordable Biosimilars
Cost of biosimilar development
Development of biosimilars begins with cloning the appropriate host cells with the target gene. This is a multistep process involving upstream processes (e.g.,…
Article
Eliminating Residual Impurities Starts with a Strategic Plan
They can be derived from the host cell or the product itself, media components, surfactants, virus inactivation agents, inorganic or organic contaminants from permanent and single-use process equipmen…
Article
QbD Improves Cell-Culture Process Control
Mammalian cells are most frequently used as the hosts for manufacturing complex biopharmaceuticals, and cultivation of these host cells is a key factor in the production of biotherapeutics. The cultiv…
Article
Platform Technologies Improve Protein Expression
“This technology has made it possible to specifically engineer genes in existing hosts to improve their properties and performance and to add completely new functionality,” he observes. There is one h…
Article
Managing Residual Impurities During Downstream Processing
Challener
Manufacture of protein and other biologic drug substances via cell culture or fermentation occurs with the natural, simultaneous production of other unwanted materials, particularly host-…