Search results for " cell lines"
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rAAV Production Using HEK293 Cell Line and Transfection Medium
Improving the performance of a scalable AAV process in Xcellerex™ XDR-10 and XDR-200 bioreactor using HyClone™ peak expression medium.
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Generating a Fully Processed Antibody
… could be used for a number of different programs, and to use the developed FGE cell line to produce cell lines and processes capable of expressing and converting high titers of tagged antibody. The …
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Impact of Media Components on CQAs of Monoclonal Antibodies
Different cell lines have different nutritional requirements because of the differences in metabolism, and this factor needs to be appropriately accounted for during media optimization. While natural …
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Downstream Processing for Cell-Based Therapies
It is, therefore, considered suitable for shear-sensitive cell lines and potentially allows for higher throughput. It also features fewer clogging issues than traditional membrane-based systems, exten…
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Single-Use Technologies Prove Effective for Viral Vector Process Development
Use of microcarriers that support adherent cell lines is also being explored as a means for scaling adherent processes in suspension in stirred-tank bioreactors.
Flatware is often used in the seed…
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Concentrating Feed—an Applicable Approach to Improve Antibody Production
These approaches did not affect product quality and were effective for multiple cell lines and applicable for large-scale manufacturing.
Recombinant protein production in the biopharmaceutical …
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Tools for Continuous Bioprocessing Development
While continuous microbioreactors are early in their development and commercial deployment, the preliminary data with microbial cell lines demonstrate the capabilities for high gas-transfer rates--sup…
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Implementation of Raw Material Control Strategies in the Manufacture of Single-Use Bioprocessing Containers
One of the degradation products, bis(2,4-di-tert-butylphenyl)phosphate (bDtBPP), inhibits growth of some Chinese hamster ovary (CHO) cell lines (8, 9, 10).
A stabilizer-degradation product impacti…
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Platform Approach Speeds Process Development
Stable cell lines are typically generated within five to seven months using highly automated standard working procedures. Thousands of clones per project are analyzed using a robotic platform includin…
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Improving Upstream Predictability
Verlenden expects to see greater use of perfusion-developed cell lines, expansion and perfusion cell-culture media, cell retention systems, single-use bioreactors developed for perfusion applications,…