Search results for " fragment"
Article
eBook: Purification of Antibody Therapeutics - Approach for Diversified Pipeline
Today, we see multispecific and bispecific antibodies, antibody fragments, and antibody drug conjugates in the development pipeline. Manufacturing platform approaches conveniently used for many tradit…
Article
Single-Step, Protein A Chromatography Process for Bispecific Antibodies in Early Screening
The goal was to separate BsAb monomer from fragments such as the individual heavy and light chain pairs while achieving high purity and yield of BsAb. MabSelect™ PrismA protein A resin was selected fo…
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Selection Guide: Columns and Resins for Antibody Purification and Immunoprecipitation
Polyclonal antibodies, monoclonal antibodies (mAbs), and antibody fragments are usually purified by affinity chromatography. Resins containing an immobilized ligand (e.g., protein A, protein G, or p…
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Development of Purification for Challenging Fc-Fusion Proteins
LMW fragment quantification was performed using a method that involves the heating of IgG samples in the presence of sodium dodecyl sulfate (SDS) under non-reducing conditions. A capillary electrophor…
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Process Chromatography: Continuous Optimization
Most chromatography vendors have developed new affinity resins designed for use with the next-generation biologic products being developed today, including antibody fragments, bispecific antibodies, a…
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Adherent Cell Culture in Biopharmaceutical Applications: The Cell-Detachment Challenge
For particular applications that are more related to research and production of cell fragments, freeze/thaw is a traditional method for cell detachment, where cell viability is not of major concern (1…
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USP Publishes Monoclonal Antibody Guidelines
Although the general chapter is focused on IgG-type mAbs, the principles of the tests included can apply to other related molecules, such as IgM or other isotype antibodies, antibody fragments, and Fc…
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PDA's Technical Report for Biotech Cleaning Validation
Although degraded fragments of the active ingredient are measured in a cleaning-validation protocol, analytical method validation is typically performed with the bulk active itself because this typica…
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A Closer Look at Affinity Ligands
The single-domain heavy chain antibody fragments from Camelidae have also been utilized successfully as affinity ligands for purification coagulation factors to help patients with hemophilia type A an…
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Detailed agenda for Chromatography + filtration virtual summit
Explore the latest advancements in process development and scale-up
Check out the full agenda below!
Join Cytiva Chromatography and filtration summit online to learn from presentatio…