Search results for "Protein A"
Article
Purifying Tagged Proteins: Learn the Basics
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What is tagged protein purification? When and why should you use it? Learn the basics in our quick guide to tagged protein purification.
Article
What If You Could Remove Your Antibody Aggregates Already in the Protein A Step?
…es: (1) elution buffer type, (2) conductivity of the elution buffer, (3) pH gradient length and (4) Protein A resin type.
Article
Rapid Cycling Protein A-Based Fiber Chromatography to Address Emerging Downstream Bottlenecks
The protein A fiber matrix has an open pore structure in which mass transfer is governed by convective flow. This structure allows high monoclonal antibody (mAb) binding capacities at very short resid…
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Tips for High Resolution Ion Exchange Chromatography to Obtain a Pure Protein
A high-resolution ion exchange chromatography run is key for obtaining high protein purity. The better the separation between peaks, the higher the purity of the proteins that will be collected.
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Single-Step, Protein A Chromatography Process for Bispecific Antibodies in Early Screening
MabSelect™ PrismA protein A resin was selected for the capture step. Most fragments could be removed as early as the capture step using gradient elution from pH 5 to 3.5, without the need for addition…
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Interpreting Protein Binding Capacities for Chromatography Resins
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Comparing the protein-binding capacities of resins from different suppliers is not straightforward because different methods may have been used and these methods are n…
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Protein Purification Handbooks
Make sure you have the latest protein purification handbooks!
Whether you are a beginner or an expert, Cytiva handbooks provide practical tips and in-depth information about protein purif…
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Simplify Every Step of Histidine-Tagged Protein Purification
A purity between 60% and 80% was achieved when using a manual purification workflow. To improve this, an automated purification approach was evaluated. Learn more in this blog post how automated his…
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Protein Characterization Using SEC: Three Webinar Takeaways
The possibility to separate molecules by size under native conditions is powerful in protein analysis. With more than thirty years in protein science, Åke Danielsson, Research Director at Cyti…
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Principles and methods for automated multistep protein purification
Principles of Automated Multistep Protein Purification
Summarized questions related to principles of multistep processing and the case study presented by Erwin van Puijenbroek and Matthias H…